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KMID : 0378019770200020123
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New Medical Journal
1977 Volume.20 No. 2 p.123 ~ p.131
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Portacaval Transpsition in Lewis Rats
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Abstract
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Many investigators were interested in the possibility that portal venous blood contained hepatotrophic factors and that the extrahepatic diversion of these factors by portacaval shunt or portacaval transposition was responsible for derangement of hepatic structure, function, and physiology. In order to assess how portal venous blood influences carbohydrate and protein metabolism and whether HPBF is responsible for hepatic deglycogenation and nucleic acid metabolism after portacaval transposition, portacaval transpositions were performed on Lewis rats weighing 200 to 300 gm by the suture technique of Lee´s original method. All animals in these experiments were sacrificed 30, 45, 50, 100, 150, 200, and 263 days subsequent to the surgical procedures.
Changes in the liver weight, as well as glycogen, DNA and protein contents in the liver of operated animals were analyzed.
The following results were obtained:
1) Decreases in the liver weight in operated animals were significant but changes in body weight was not significant.
2) The total glycogen decreased an average 41.2 percent. The principal loss was in the TCA soluble glycogen, the mean decrease being 52. 0 percent. TCA insoluble glycogen decreased an average of only 6%. The changes in total and TCA soluble glycogen were significant. The. change in TCA insoluble glycogen was not significant.
3) The contents of DNA in the liver of transposed animals increased at 100 days after operation. This result indicate that the size of cells decreased, although the numbers of liver cells. remained unchanged.
4) Slight decrease in protein contents in the livers of portacaval transposed animals suggested. loss of cell parenchym associated with liver atrophy.
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